Determination Of The Agnor Parameters, Mn Frequency, Anae And Acp-Ase Positivity Of Pbl In Pregnants

This study was performed to determine the effects of pregnancy on the positivity rates of alpha- naphthyl acetate esterase (ANAE) and acid phosphatase (ACP-ase) of the peripheral blood lymphocytes (PBL) and PBL percentages in pregnant women. Micronucleus frequency (MN) and some AgNOR parameters of PBL were also estimated. Women divided into four groups as Control; Trimester I (TRI); Trimester II (TRII) and Trimester III (TRIII) (n= 10 for each group). ANAE and ACP-ase demonstrations, modified May-Grünwald Giemsa and AgNOR staining were performed on blood smears prepared from peripheral blood samples. The lowest T-lymphocytes percentage (58,2%) was determined in TRII and the differences between the groups were statistically important (p<0,05). There was a statistically significant decrease in the proportions of the ACP-ase (+) lymphocytes in TRI (57,9%) and TRIII (57,8%). A dramatic reduction in the percentage of PBL was recorded during pregnancy (p<0,05), whereas the highest null cell rates (11%) were found in TRI. A distinct increasing was observed in MN frequency during pregnancy (p<0,05). There was no difference in mean AgNOR counts between the groups, whereas AgNORs area/nucleus area increased during pregnancy and it gained its highest value (10,22%) in TRIII. It was concluded that the ANAE- and ACP-ase positive PBL proportions, PBL percentages, MN frequency and some AgNOR parameters were affected by pregnancy although there were some differences among the TRs.