To determine human sperm cell motility and DNA integrity exposed to mobile phones broadcasting at 900 MHz and 1800 MHz frequency bands according to different distances. Two main groups were created with each containing 20 samples. Samples were treated for 15 minutes with 900 MHz and 1800 MHz radiofrequency actively emitted radiation. Each group was divided into three subgroups as; control group, 2,5 cm and 10 cm distance exposed group. Each group was evaluated in terms of sperm motility (according to WHO 2010 criteria) and DNA integrity (Acridine Orange Staining). Statistically the motility parameter (Class A+B type) of 2,5 and 10 cm radiation exposed groups showed no any significant difference for both 900 MHz and 1800 MHz frequency bands (p>0,05). But for comparisons of DNA integrity there was a statistically significant difference for groups related inversely with the distance (p<0,05). Our data reveals that reduction of the distance from the source of radiation increases the rate of damage of DNA integrity. Therefore it can be suggested that it is as safer as one keeps mobile phone as long away from his/ her body.