Effects Of High-Fat Diet And Acrylamide On Tissue Oxidant And Antioxidant Levels In Rats

Acrylamide (ACR) is an organic chemical which occurs in foods extensively consumed in diets worldwide. ACR promotes the generation of reactive oxygen species and the depletion of antioxidants. The aim of this study was to investigate liver, brain and kidney of tissue total antioxidant status (TAS), total oxidant status (TOS) and liver and brain of tissue oxidized LDL (ox-LDL) levels in long term ACR + standard diet and ACR + high-fat diet given rats, compared to control rats. Forty-eight male Wistar rats (5-6 weeks of aged) were segregated into two diet groups and fed with a high-fat diet (crude fat 20%) or standard diet (crude fat 2.7%) respectively; and animals in each diet groups were exposed to acrylamide at the dose of 0, 2, 10 and 20 mg/kg bw/day via drinking water for 28 days. At the end of the experiment tissue samples were analyzed for TAS, TOS and ox-LDL. ox-LDL and TOS levels were increased as doses of acrylamide were elevated in liver and brain tissue, no significant difference was present at levels of ox-LDL/protein (p=0.087) and TOS/protein (p=0.751) in liver tissue among eight groups. While no significant difference was observed at levels of ox-LDL/protein in brain tissue (p=0.808) among eight groups, TOS/protein levels in brain tissue (p<0.001) became significantly increased. No significant difference was present at levels of TOS/protein (p=0.052) in renal tissue among eight groups. As doses of acrylamide were increased, TAS/protein levels in brain, liver and renal tissue became decreased, and a significant difference was present at TAS/protein levels among the groups (p<0.001). Our findings showed that long term treatment with 2, 10 and 20 mg/kg doses of ACR + standard diet and ACR + high-fat diet treatment led to a significant depletion of tissue TAS levels and over production of tissue TOS and ox-LDL levels, consequently, to an increase in oxidative stress.