Determination Of The Agnor Parameters, Mn Frequency, Anae And Acp-Ase Positivity Of Pbl In Pregnants

BAKİ AKBULUT, EMRAH SUR, DÜRDANE NİL OKUR

  • Year : 2015
  • Vol : 31
  • Issue : 4
  •  Page : 144-150
This study was performed to determine the effects of pregnancy on the positivity rates of alpha- naphthyl acetate esterase (ANAE) and acid phosphatase (ACP-ase) of the peripheral blood lymphocytes (PBL) and PBL percentages in pregnant women. Micronucleus frequency (MN) and some AgNOR parameters of PBL were also estimated. Women divided into four groups as Control; Trimester I (TRI); Trimester II (TRII) and Trimester III (TRIII) (n= 10 for each group). ANAE and ACP-ase demonstrations, modified May-Grünwald Giemsa and AgNOR staining were performed on blood smears prepared from peripheral blood samples. The lowest T-lymphocytes percentage (58,2%) was determined in TRII and the differences between the groups were statistically important (p<0,05). There was a statistically significant decrease in the proportions of the ACP-ase (+) lymphocytes in TRI (57,9%) and TRIII (57,8%). A dramatic reduction in the percentage of PBL was recorded during pregnancy (p<0,05), whereas the highest null cell rates (11%) were found in TRI. A distinct increasing was observed in MN frequency during pregnancy (p<0,05). There was no difference in mean AgNOR counts between the groups, whereas AgNORs area/nucleus area increased during pregnancy and it gained its highest value (10,22%) in TRIII. It was concluded that the ANAE- and ACP-ase positive PBL proportions, PBL percentages, MN frequency and some AgNOR parameters were affected by pregnancy although there were some differences among the TRs.
Cite this Article As : Akbulut B,Sur E,Okur ND.Gebelerin Kan Lenfositlerinde AgNOR ve MN Değeri ile ANAE ve ACP-az Pozitivitelerinin Belirlenmesi,Selçuk Med J 2015;31(4): 344-350.

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Description : None of the authors, any product mentioned in this article, does not have a material interest in the device or drug. Research, not supported by any external organization. grant full access to the primary data and, if requested by the magazine they agree to allow the examination of data.
Determination Of The Agnor Parameters, Mn Frequency, Anae And Acp-Ase Positivity Of Pbl In Pregnants
, Vol. 31 (4)
Received : 19.12.2014, Accepted : 19.12.2014, Published Online : 13.08.2018
Selçuk Tıp Dergisi
ISSN:1017-6616;
E-ISSN:2149-8059;