Effects Of High-Fat Diet And Acrylamide On Tissue Oxidant And
Antioxidant Levels In Rats
ÜMMÜGÜLSÜM CAN, HARUN PERU, SERKAN ÇAKIR
- Year : 2016
- Vol : 32
- Issue : 2
- Page :
38-42
Acrylamide (ACR) is an organic chemical which occurs in
foods extensively consumed in diets worldwide. ACR promotes
the generation of reactive oxygen species and the depletion of
antioxidants. The aim of this study was to investigate liver, brain and
kidney of tissue total antioxidant status (TAS), total oxidant status
(TOS) and liver and brain of tissue oxidized LDL (ox-LDL) levels in
long term ACR + standard diet and ACR + high-fat diet given rats,
compared to control rats. Forty-eight male Wistar rats (5-6 weeks of
aged) were segregated into two diet groups and fed with a high-fat
diet (crude fat 20%) or standard diet (crude fat 2.7%) respectively;
and animals in each diet groups were exposed to acrylamide at the
dose of 0, 2, 10 and 20 mg/kg bw/day via drinking water for 28 days.
At the end of the experiment tissue samples were analyzed for TAS,
TOS and ox-LDL. ox-LDL and TOS levels were increased as doses
of acrylamide were elevated in liver and brain tissue, no significant
difference was present at levels of ox-LDL/protein (p=0.087) and
TOS/protein (p=0.751) in liver tissue among eight groups. While
no significant difference was observed at levels of ox-LDL/protein
in brain tissue (p=0.808) among eight groups, TOS/protein levels in
brain tissue (p<0.001) became significantly increased. No significant
difference was present at levels of TOS/protein (p=0.052) in renal
tissue among eight groups. As doses of acrylamide were increased,
TAS/protein levels in brain, liver and renal tissue became decreased,
and a significant difference was present at TAS/protein levels among
the groups (p<0.001). Our findings showed that long term treatment
with 2, 10 and 20 mg/kg doses of ACR + standard diet and ACR
+ high-fat diet treatment led to a significant depletion of tissue
TAS levels and over production of tissue TOS and ox-LDL levels,
consequently, to an increase in oxidative stress.
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Description :
None of the authors, any product mentioned in this article,
does not have a material interest in the device or drug. Research,
not supported by any external organization.
grant full access to the primary data and, if requested by the magazine
they agree to allow the examination of data.
Effects Of High-Fat Diet And Acrylamide On Tissue Oxidant And
Antioxidant Levels In Rats
2016,
Vol.
32
(2)
Received : 03.09.2015,
Accepted : 03.09.2015,
Published Online : 13.08.2018
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ISSN:1017-6616;
E-ISSN:2149-8059;