Efficacy of Low Dose N-Acetylcystein Theraphy on Experimental Rabbit Sepsis Model

Purpose: To determine the effect of NAC therapy on plasma levels the protective role of tissue injury and organ dysfunction of free oxygen radicals in experimental sepsis model. Material and method: 30 female rabbits were divided into 3 groups, each composed of 10 rabbits. Sepsis was constituted by caeucum ligation perforation tecnique. Groups named sham, sepsis and sepsis + NAC (10mg/kg/day) were formed and medication was given 12 and 24 hours after caeucum ligation and perforation. At the same time blood samples to measure blood gases, plasma glutathion (GSH), malondialdehyde (MDA) and biochemical diagnosis were collected. Tissue samples were obtained. Findings: Plasma GSH levels were elevated in NAC group with respect to sepsis group (P<0.05). Plasma MDA levels were decreased in sepsis group when compared to NAC group (P<0.05). Liver tissue MDA levels were found to be same in both groups. AST, ALT and creatinine values were elevated significantly in NAC group when compared to sepsis group (P<0.05). Apoptosis in liver tissue was significantly decreased in NAC group when compared to sepsis group (P<0.05). Degree of mononuclear infiltration in kidneys were decreased in NAC group when compared to sepsis group (P<0.05). Conclusion: Application of low dose NAC an antioxidative agent in experimental sepsis model has beneficial effects on pulmonary functions and plasma MDA, GSH levels. More and comprehensive studies which tests different NAC doses are needed to determine the role for NAC in experimental sepsis model.