Evalution Of The Puma And P53 Significance In Pathogenesis Of Multiple Myelom

\n Introduction: Multipl myeloma is a disease of unknown pathogenesis. Although there was a different

\n hypotheses on the subject, recently mutations in the pathway of apoptosis come to the fore. PUMA may play a role in apoptosis, bound or

\n unbound p53. The role is regulated by various signals of transcription factors. In this study; the expressions of p53 and PUMA in bone marrow hematopoetic cells of

\n have been worked and the relationship between myeloma pathogenesis with p53- PUMA was

\n searched.

\n Method: The expression of p53 and PUMA was evaluated using immunohistochemistry. The

\n procedure was carried out using specific antibodies against p53 and PUMA. Bone marrow

\n biopsies of MM patients at the time of diagnosis (n=31) were included in the study group.

\n Bone marrow biopsies from individuals who had normal hematopoiesis (n=12) were included

\n as control. After staining, strength was calculated of each positively staining

\n cells.

\n Findings: The percentage of cells showing PUMA expression was significantly higher in

\n bone marrows of MM patients as compared to normal bone marrow samples (p=0.000). We

\n found a correlation between the percentage of plasma cells with staining prevalence of

\n PUMA, but it was not statistically significant (p=0.385). There was no correlation between

\n the stage of the disease with PUMA expression.

\n Results: Our results showed that specially PUMA expression is significantly higher in bone

\n marrow cells of MM patients compared to healthy controls. Higher positivity of PUMA

\n suggested that, PUMA induced nongenotoxic reasons at the time of diagnosis. The lack of

\n p53 and PUMA positivite suggests that, apoptosis play a role in myeloma pathogenes