Light Microscopic Investigation of Stabilized Nasal Mast Cells of Rats

In this light microscopic study, the effects of cromolyn sodium on nasal mast cells of rat was itıvesiigated using alcian blue-safranin O and toluidin blue staining methods. The investigation was peıformed on 20 male and female albino rats which were obtained from the Experimental Animal Laboratory of the Faculty of Medicine, Selçuk University. Rats were divided into two groups of ten rats each. First group was considered as control group. Cromolyn sodium was administered to the second group. Rats were anesthetized by ether and regio respiratoria nasi of rats were frontally excised. Tissues were fixed in Carnoy solution for three days at room temperature, dehydrated with absolute alcohol and embedded in paraffin. Five micrometer sections were cut from the paraffin blocks and stained with alcian blue-safranin O and toluidin blue solutions. frı conclusion, this study revealed that, as an inhibitory agent, cromolyn sodium is ineffective on nasal mucosal most cells but significantly effective on connective tissue mast cells.