The Effect Of Ischemia Reperfusion Injury After Haemorrhage On Liver Oxidant-Antioxidant Status

Aim: Various types of haemorrhage can cause hypovolemic shock. Volume replacement for prevention of hypovolemic shock results with reperfusion. The aim of this study was to determine the hepatic tissue MDA, XO, SOD and AOA levels after reperfusion following ischemia with duration of 30 minutes. Method: Twenty four female Spraque-Dawley rats were divided into four groups of six rats in each. Group A was the control group and B, C, and D groups were ischemia-reperfusion groups which were reperfused for 1 hour, 3 hours and 24 hours after 30 minutes hemorrhagic ischemia, respectively. In the ischemia-reperfusion groups at the end of the reperfusion periods, rats were sacrified and the liver tissue samples were collected. In group A rats were only anesthetized for one hour and then they were sacrified and the liver tissue samples were collected. MDA, XO, SOD and AOA analyses were performed in liver tissues. Results: MDA and XO levels were significantly increased in the ischemia- reperfusion groups when compared with the control group (p<0,05). These changes were most obvious in the first hour. Conclusion: Our findings showed that volume replacement therapy after hypovolemic shock was lead to reperfusion injury. Because MDA, XO, SOD ve AOA changes were more significant in the first hour of the reperfusion than the 3th and 24th hours; we suggest maintenance of antioxidant suplements with replacement theraphy to decrease the reperfusion injury. For the supplemental management antioxidants such as alpha tocopherol, ascorbic acid and melathonin can be prefered in the first hour of reperfusion especially.