The Investigation Of Cryptosporidium Spp. In Patients With Diarrhea
By Microscopic And Elisa Methods
AHMET YILMAZ, ÖNDER AKKAŞ, MERAL BAYAR, HAKAN USLU, KEMALETTİN ÖZDEN
- Year : 2016
- Vol : 32
- Issue : 3
- Page :
61-64
Cryptosporidium spp. has still an important place among
parasites which are the main reason of diarrhea both developing and
developed countries. It is an obligate intracellular protozoan that may
lead fatal diarrhea in immune-suppressed persons. In this study, our
aim was to investigate the presence of Cryptosporidium oocysts as
an agent in pre-diagnosed patients with chronic and acute diarrhea
by using both Modified Acid Fast Staining and ELISA methods. For
our study, 86 diarrheal stool samples were collected which were sent
from Ataturk University Medical Faculty Research and Application
Hospital Clinics to Microbiology Laboratory between December 2013
and May 2014. At the same day, Modified Acid Fast Staining method
was performed to stool samples and examined under the microscope
(with 100X magnification). A small pieces of feces were placed in
eppendorf tubes without adding any preservatives and stored at
-200
C. After a while, ELISA method was performed to these stored
samples for detecting Cryptosporidium antigens. Kit was performed
as recommended by the manufacturer and the results was evaluated
with ELISA reader at 450 nm. A total 6 of samples by Modified Acid
Fast Staining and 8 samples by ELISA were detected as positive in
stool samples of 86 patient (43 female and 43 male) having age range
of 0-83 years. Four (50%) positive samples, which were detected by
ELISA, were belong to either female or male patients. For Modified
Acid Fast Staining, 2 (33.3%) of positive samples were belong to
male patients, 4 (66.7%) of positive samples were belong to female
patients. As a consequent; although ELISA method is costly than
staining method, we suggest that ELISA method should be preferred
for Cryptosporidium diagnosis because of the advantages including
quick results and ease of application.
Cite this Article As :
Download Citation: Endnote/Zotero/Mendeley (RIS) RIS File
Download Citation: BibTeX BibTeX File
Description :
None of the authors, any product mentioned in this article,
does not have a material interest in the device or drug. Research,
not supported by any external organization.
grant full access to the primary data and, if requested by the magazine
they agree to allow the examination of data.
The Investigation Of Cryptosporidium Spp. In Patients With Diarrhea
By Microscopic And Elisa Methods
2016,
Vol.
32
(3)
Received : 21.12.2015,
Accepted : 21.12.2015,
Published Online : 13.08.2018
Selçuk Tıp Dergisi
ISSN:1017-6616;
E-ISSN:2149-8059;