Makale Arama

Aim: Brucellosis, a zoonotic disease, may affect several body systems and cause various clinical manifestations considering the infection sites. Diagnosis of brucellosis is mainly based on culture and serological methods, specifically Rose Bengal agglutination (RBT) and standard tube agglutination tests (STA). When RBT, which is usually used as a screening method, is positive for Brucella antigens, STA is prefered to detect an agglutination titre by using serial dilutons of serum sample. On the otherhand, false negative results can be obtained by STA due to the presence of blocking antibodies. The refore, these two methods may be in adequate for diagnosis of Brucellosis. The aim of this study is to compare a novel method, Brucella Coombs gel test (BCGT) with other four serological methods, RBT, STA, Brucella immune-capture agglutination test (BCAP), and Brucella ELISA IgG, IgM tests. Patients and Methods: Serum samples taken from 100 patients, admitted from various clinics at Konya Training and Research Hospital were sent to the Medical Microbiology Laboratory with a clinical diagnosis of Brucellosis. Each serum sample was studied with RBT (Seromed Laboratory Products, Turkey), STA (Biomedica, Canada), BCAP (Brucellacapt, Vircell SD Spain), BCGT (ODAK, ISLAB, Turkey), and ELISA Brucella IgG, IgM (Euroimmune, Germany) methods. All procedures were carried out in accordance with the manufacturer’s recommendations. Results: The rates of positive results for each method were as follows: BCGT 88 (88.0%), RBT 74 (74.0%), STA 56 (56.0%), BCAP 84 (84.0%) and Brucella IgG, IgM test 92 (92.0%). Conclusion: Statistical analysis could not be executed due to lack of a gold standard method in the study. Yet, BCGT provided faster results than STA and BCAP methods because it does not require a 24-hourincubation. This novel test, BCGT, also showed an advantagein the diagnosis of Brucellosis because of detecting blocking antibodies. More comprehensive studies are needed to be performed to confirm the results.

  Brusellosis is a zoonotic disease which is transmitted with nonpasteurized milk and milk products. Mostly effects the gastrointestinal system and less the genitourinary system. Most common genitourinary complication of brucellosis is epididymo-orchitis.

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 Here we present a case which presented with right lower quadrant pain in which physical examination lead us to the preliminary diagnosis of appendicitis after laboratuary results, cilinical findings and deepining the history the patient was diagnosed with unilateral epididimoorchit and here presented with clinical laboratuary findings. Brusellosis which is endemic in our country, should be kept in mind especially when accompanied with acute abdomen eventhoug imaging prosedures do not confirm the diagnosis. 

Brucellosis is a zoonotic disease caused by bacteria of the Brucella subspecies from the gram-negative bacteria family. Brucellosis continues to be a public health problem worldwide, especially in developing countries. The bacteria can involve the reticuloendothelial system foremost, and many systems such as joints, the heart and the kidneys. Despite the respiratory system involvement being known, lung involvement is rare. Non-specific findings and complications in the patients with lung involvement makes the diagnosis difficult. In this case report, a 6-year-old male patient, presenting with the complaints of fever, cough, expectoration, hemoptysis, exhaustion, anorexia and knee ache, was hospitalized with the diagnosis of pneumonia and pleural effusion. On the performed examinations, hepatosplenomegaly and bicytopenia were detected. The Rose-Bengal test was (+++) and the Wright agglutination test was 1/1280 (+) in the blood and pleural effusion fluids of the case. The case was considered to be a pneumonia and pleural effusion due to brucella. The case has been presented since it is rarely seen, and in order to emphasize that in countries such as ours, in which brucella is endemic, complications due to brucella show a wide spectrum.

On a group of patients which were sended la aur laboratories and swpected of having brucellosis who were admitted to our hospital's iR patieni and out paıient Wright aggluiination tesis (WAT), Rose Bengal Tesis (RBT), and SİR, tesis (ST) were performed and evrıluaıed fr r Bruce11a abor While ST and RIIT were negative, WAT was 1140 positive, in a patient's serum. RBT was posi-tive, and WAT was 1140 positive, ha ve heen found, in anoıher serum. ST and RBT were positively correlated with 1180 and higher dilutions of WAT (100 per ccnt).

Milk is an ideal and natural drink that contains all the vital nutrient flour-wastes necessary for the organism to grow and survive.

Aim: Many serological tests are used in the diagnosis and follow-up of Brucellosis. Those routinely used in laboratories are Rose Bengal Aglutination, Standart Tube Aglutination and ELISA anti-Brucella tests. In addition to these tests, available Brucellacapt (immuncapture) aglutination and coombs tests are also used in the diagnosis. In this study, Rose Bengal Aglutination, Standart Tube Aglutination, Brucellacapt and ELISA tests were aimed to be compared. Method: Sent from various clinics with the prediagnosis of Brucellosis, the samples of 71 patients were investigated in the study. These samples were investigated using Rose Bengal, Standart Tube Aglutination, Brucellacapt and ELISA (Brucella-IgM, Brucella-IgG) tests. Results: Fifty six positivity rates were determined with Rose Bengal Aglutination test (78.8 %), 30 with STA (42.2 %), 52 with Brucellacapt (73.2 %) and 58 with ELISA (81.6 %). Conclusion: It was considered that STA is unsatisfactory in the diagnosis of Brucellosis in the patients for whom no culture could be performed, when performed alone, and that Brucellacapt and/or ELISA tests are necessary to be used combinedly. However, in the follow-up of Brucellosis, it was also concluded that Brucellacapt is inappropriate; instead, the use of ELISA (IgM and IgG) tests will be more appopriate in the diagnosis of Brucellosis.

Introduction and Objective

Brucellosis is a zoonotic infection seen as an endemic in Turkey and is a public health problem at the same time. The objective of this study was to evaluate demographic/epidemiologic, clinic, laboratory features, complications and treatments in brucellosis patients followed-up in our clinic.

Material & Methods

In this study, features of patients followed-up between 01/01/2010 and 31/12/2018 in our clinic were retrospectively evaluated. Descriptive data were expressed as number and percentage. Categorical variables were analyzed with Chi-square test and numerical variables with Student’s t test.

Results

Of the total of 365 brucellosis patients, 159 (43.56%) were female and 206 (56.44%) were male. The mean age of the patients was 45.9±14.51 (18-82) years. The most common time of presentation was summer season with 137 (37.5%) patients. The most common transmission route of the disease was a history of animal husbandry with 252 (69%) patients. Of all cases 168 (46%) were acute, 96 (26.3%) were subacute, and 101 (27.7%) were chronic brucellosis. The most common complaint of the patients was fatigue in 302 (82.7%) patients with being higher in acute brucellosis (p=0.0015). Anemia was found in 53 (33.3%) male and 114 (55.3%) female patients with being significantly higher in female patients (p=0.0283). Leukopenia was found in 7.9%, leukocytosis in 16.2%, thrombocytopenia in 9.6%, neutropenia in 4.1%, neutrophilia in 9% and lymphomonocytosis in 12% of the patients. Wright agglutination test was performed in 26 (7.1%) and brucella immunocapture agglutination test in 361 (98.9%) patients and all results were positive. Of all patients, 172 (47.1%) developed complications with spondylodiscitis being the most commonly found in 58 (15.9%) patients. Sixty-one (31.8%) of patients developed relapse.

Conclusion: Since brucellosis is endemic in our country, it should be considered in presumed diagnosis of patients presenting with complaints such as fatigue, articular pain and fever.

Brucellosis is a bacterial zoonotic disease with the major symptoms such as fever, joint and muscle pain generally caused by consumption of meat and milk of infected animals. Serological test methods such as Rose Bengal plate agglutination (RBPA) and standart tube agglutination test, are used in the diagnosis of brucellosis because of the lack of availability of blood culture in several laboratories, the low growth rate and ease to be effected by antimicrobials of the bacteria. Variations in biochemical parameters could be seen in the course of infection. In this study, seroprevalence of brucellosis in our region was determined by Rose Bengal and standart tube agglutination tests, and variations in biochemical parameters of the sera defined as positive for brucellosis were also evaluated. Rose Bengal test was found to be positive for 144 out of 4041 patient sera send to Microbiology Laboratory with the initial diagnosis of brucellosis. Among these sera, agglutination titer of 1/160 and over was detected in 121 samples. Seroprevalence for brucellosis was 3.56% for our study group. Among patient sera found to be positive in STA, 98 (81%) showed increase in C-reaktif protein (CRP) level, 66 (54.5%) in erytrocyte sedimantation rate (ESR), 29(24%) in alanin aminotransferase (ALT) and aspartat aminotransferase (AST). Additionally, leucocytosis was seen in 11 (9.1%), leucopenia in 12 (9.9%), anemia in 46 (36%) and thrombocytopenia in 53 (43.8%) patient sera. As STA titer increased CRP level had a paralel rise, but the platelet and leukocyte number decreased. In conclusion, especially serum CRP level could be a useful biochemical test parameter for the diagnosis and follow-up of brucellosis.

We report a patient with pancytopenia which is an unusual manifestation and a rare hematologic complication of acute brucellosis. A 29-year-old female patient was hospitalized with fever and pancytopenia. Brucella standard tube agglutination test was found to be positive and cultures from blood yielded growth of Brucella melitensis. The patient completely recovered by the sixth week following combined antibacterial treatment of doxycycline and streptomycine. Brucella should be considered as an uncommon cause of pancytopenia and should be treated immediately.

Brucella infection may produce a variety ofnon-spesific haematological abnormalities. Bone mar-row and spleen are commonly involved in this in-fection and such involvement may result in a pane-topenia. In this arcticle. a pancytopenia case in brucellosis has been reported. Pancytopenia re-solved on treatment of the brucella infection. We want to stressed the importance of ha-ematological complications for brucellosis Ltiith this case.

Brucellosis is one of the most frequent zoonotic diseases in our country. Brucellosis, a systemic infection, can arise with various clinical involvements. Genitourinary involvement is seen at varying rates between 4-20%.Seven examinedepididymo-orchitis cases were diagnosed through anamnesis, physical examination and ultrasonography.All of our patients are provincials, in their anamnesis, there are histories regarding eating infected milk and dairy products and         miscarriages in animals. Only in two of seven cases, diagnosis was made through coombs tube agglutination; in the other five of seven cases, standard tube agglutination test was sufficient for diagnosis. Blood culture positiveness was determined in three patients. Patients were healed by taking brucella treatment for six weeks.      Combined modality therapy was administered to all patients. Treatment deficiency was not determined in any patients. In this article, we aim to provide taking into consideration also brucellosis in histories of patients having epididymo-orchitis treatment.

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