Makale Arama

Background: Electromagnetlc fields (EME) are the most important environment problems that effect the public health in our century. Increasing incidence of coronary heart disease, leukemia, lymphoma, and Alzheimer in the patients who are effected from magnetic fields, are revealing that these sources can not be used unlimitedly. İn this study, we would like to investigate the effect of hairdryer on the rats. Materials and methods: İn this study, 200-250 gr weighted 29 male rats vvere used. 15 of them were control group, and 14 vvere faced with EMF. A BKK 1161 SK hairdryer was used as the magnetic field source. This machine had 600 VVatt (W) power, 220 V - 50 Hz resistance, and a mean magnetic field counted 100-150 mG. The distance betvveen the subjects and the machine was 20-25 cm. EMF was performed on them for 3 days of each week, and 5 minutes of each day, and it was lasted after 3 months with a total period of 205 minutes. At the end the malondialdehid (MDA) levels in the subjects sera vvere calculated, and they vvere sacrificed and their cerebrum, thymus, lungs, heart and great vessels, liver, kidneys, and spleen vvere taken to histopathologic examination under light microscope. Results: İt was obviously observed that the EMF in 50 Hz freguency increases the mononuclear celi infiltration in the viscera; especially, it was estimated that EMF causes alveolar destruction in the lungs, tubuler degeneration in the kidneys, glial proliferation in the cerebrum, fibrosis in the liver, and adventitial mononuclear celi infiltration in the great vessels in 3 months, vvhich is thought to be a very short period. Statistically, the subendothelial dissection in the great vessels was not found significant, hovvever, this was thought to be an important finding. The serum MDA levels of EMF group vvas increased, but statistically, this was not significant. Conclusion: We concluded that EMF causes an increase in the production of free oxygene radicals, and in the late-term this results with various diseases including cancers and atherosclerotic dişe ases.

The excessive maternal alcohol drinking results in Fetal Alcohol Syndrome which is a pathology caused by the embryotoxic effects of ethanol in prenatal period. The mechanisms of ethanol action at the cellular or molecular levels are scarce. In this study it was aimed to investigate the effects of ethanol on growth and development of mammalian embryos as well as the morphological structure and the role of free radicals on these effects. Wistar rats were obtained from the Selcuk University Experimental Medicine Research Center and their 9.5 days embryos were explanted and cultured for 48 hours in rat serum. Whole rat serum used as a culture medium fort he control group while different concentrations of ethanol (250-500 mg%) were added tor at serum fort he experimental groups. Also, the lowest effective concentration of ethanol for all parameters was added to the culture media in the presence of an antioxidant superoxide dismutase (SOD). Ten embryos were used for each experimental condition. Dose-dependent effects of ethanol on embryonic developmental parameters such as; total morphological score, yolk sac diameter, crown-rump length, somite number, embryo and yolk sac protein contents were compared using morphological and biochemical methods. Each embryo was evaluated for the presence of any malformations. Compared to the control embryos, ethanol significantly decreased all developmental parameters döşe-dependently (p<0.05) with an increase in overall dismorphology. The haematoma in different regions, especially in the neural tube was most frequently observed. When the SOD was added to theculture media in the presence of ethanol, growth and developmental parameters were improved (p<0.05) and there was a decrease in the incidence of malformations (p<0.05). A döşe dependant developmental toxicity of ethanol on rat embryos during organogenesis wasdetermined, these effects might involve free oxygen radicals.

Objective: Nitric oxide (NO) and free oxygen radicals have been implicated in the pathogenesis of intestinal circulatory dysfunction in sepsis. The aim of this study is to investigate the effects of pentoxyfillin, L-arginin and Aminoguanidine on plasma malondialdehide (MDA) and nitric oxide (NO) levels, and to determine the relations between MDA and NO levels on intestine pathology. Methods: 60 Wistar Albino rats were used in this study. The rats were divided into 6 groups, each containing 10 subjects. Sepsis was induced by cecal ligation and puncture (CLP) method. Group I: Sham group, Group II: CLP (sepsis), Group III: CLP + 10 mg/kg L-arginin admiministration, Group IV: CLP + 15 mg/kg Aminoguanidine administration, Group V: CLP + L-arginin + Aminoguanidine (as groups III and IV) Group VI: CLP + 15 mg/kg/day pentoxyfillin. Blood samples were taken fort he determination of NO, MDA, leukocyte counts, a segment of ileum tissue sample was obtained for determination of tissue damage. Results: Leukocyte count increased in CLP induced groups significantly. While NO levels were significantly higher in sepsis and L-arginin groups the levels in Aminoguanidine and Aminoguanidine + L-arginin groups were similar to Sham group. Intestine tissue damage in sepsis and L-arginin groups were more severe than the other groups. MDA levels in CLP induced groups were found to be higher than the sham group. Conclusion: Aminoguanidine and Pentoxifillin may be added to treatment protocols in sepsis in order to prevent intestinal tissue damage and dysfunction both of which (at least partially) caused by elevated NO levels.

Objective: Oxidative stress is defined as the event that the concentration of reactive oxygen species (ROS) formed in tissue or cells exceeding the antioxidant capacity. Long duration oxydative stress predisposes to cancer development. In this study, the role of oxydative stress in colorectal cancers was searched. Material and Methods: 17 patients withcolorectal cancer operated in Ankara University, Faculty of Medicine, Department of Surgical Oncology and Ankara Numune Training and Research Hospital between 2000-2001 were included in the study. During the operation, 1cm3 of mucosa was harvested from the tumoral and neighbouring healthy tissue just following the extraction of specimen. Malondialdehyde (MDA), Superoxide dismutase (SOD), Catalase (CAT) and Glutatione peroxydase (GPX) levels were measured in these tissue samples. Results: Concentration of free oxygen radicals in tumoral and healthy normal tissues were found to be statistically non-different. Conclusion: Oxidative stress in tumoral tissue of colorectal cancers was not different from the normal tissue.

Purpose: To determine the effect of NAC therapy on plasma levels the protective role of tissue injury and organ dysfunction of free oxygen radicals in experimental sepsis model. Material and method: 30 female rabbits were divided into 3 groups, each composed of 10 rabbits. Sepsis was constituted by caeucum ligation perforation tecnique. Groups named sham, sepsis and sepsis + NAC (10mg/kg/day) were formed and medication was given 12 and 24 hours after caeucum ligation and perforation. At the same time blood samples to measure blood gases, plasma glutathion (GSH), malondialdehyde (MDA) and biochemical diagnosis were collected. Tissue samples were obtained. Findings: Plasma GSH levels were elevated in NAC group with respect to sepsis group (P<0.05). Plasma MDA levels were decreased in sepsis group when compared to NAC group (P<0.05). Liver tissue MDA levels were found to be same in both groups. AST, ALT and creatinine values were elevated significantly in NAC group when compared to sepsis group (P<0.05). Apoptosis in liver tissue was significantly decreased in NAC group when compared to sepsis group (P<0.05). Degree of mononuclear infiltration in kidneys were decreased in NAC group when compared to sepsis group (P<0.05). Conclusion: Application of low dose NAC an antioxidative agent in experimental sepsis model has beneficial effects on pulmonary functions and plasma MDA, GSH levels. More and comprehensive studies which tests different NAC doses are needed to determine the role for NAC in experimental sepsis model.

İn this study the role ofoxygen free radicals in the etiopathogenesis of Acute Rheumatic Fever (ARF) in childhood and its relationship betvveen the clinical fındings were investigated. Tvventy three patients with an age range of 5 to 15 years and 25 healthy children were included. Carditis was observed in 17 of the patients. The ROM levels were determined at the time of diagnosis and periodically after than. Oxygen free radicals and its products were measured colorimetrically using dROM's kit (d-ROMs test, Diacron s.r.l. Diagnostics Division, Via Zircone n. 8-58100 Grosseto-İtaly). The plasma ROM level at the time of diagnosis compared to that of control group was found statistically significant (P<0.05). We observed a Progressive decrease in plasma ROM levels at days 15, 30 and 90. The patients with carditis didn't have statistically different plasma ROM levels compared to the patients without carditis. The plasma ROM level at day 90 was stili higher, though unsignifıcant, than that of the control group. İn this study, we conclude that the oxygen free radicals may play an important role in the etiopathogenesis of ARF.

Purpose: The aim of this study was to detect the effectiveness of mannitol’s antioxidant capacity in the treatment of ischemia-reperfusion injury after acute Peripherie arterial occlusion. Material and Methods: 20 healthy individuals and 32 patients with acute arterial occlusion were taken to study. Of the acute isehemia diagnosed patients, Standard surgery was performed on 17 patients and surgery and mannitol infusion were given to 15 patients. Blood samples were taken from the deep veins and MDA, CPK, GSH-PX levels were measured in the acute isehemia diagnosed period and 120th minutes of the reperfusion. Results: MDA of mannitol treatment group in the isehemie period meaningfully inereased in consideration with the control group. İn consideration of reperfusion period with isehemie group in mannitol treatment MDA signjficantly decreased. İn the reperfusion period of mannitol treatment GSH-PX significantly inereased according to control group. Although the CPK results showed significant inerease in each period of both treatments, the reperfusion period of mannitol treatment showed a significant decrease in CPK considering with it’s isehemie period. Conclusion: İn acute arterial occlusions it was shown that mannitol decreases the activity of free oxygen radicals, inereases the antioxidant capacity, and decreases the tissue injury meaningfully.